er-tr7 ab antibody  (Cedarlane)

Journal: Science Advances

Article Title: Retracing the evolutionary emergence of thymopoiesis

doi: 10.1126/sciadv.abd9585

Figure Lengend Snippet: ( A ) Representative flow cytometric analyses of Ly51 expression and UEA1 binding on TECs for mice; wt ( n = 22), Cm_Foxn4 ( n = 6), Cm_Foxn1 ( n = 11), and Cm_dtg ( n = 5). ( B ) Epithelial microenvironment of reconstructed thymi resolved by keratin 5 (K5) (in green) and K8 (in red) staining; 4-week-old mice. m, medulla; c, cortex. Note the small size of the Cm_Foxn4 -reconstructed thymus. ( C ) Differential gene expression patterns in TEC transcriptomes relative to wild-type mice; Bl_Foxn4 ( n = 4), Cm_Foxn4 ( n = 4), and wt ( n = 3). ( D ) Localization of Aire + cells relative to cortical (K8) and medullary (K5) areas. ( E ) Differential gene expression patterns in TEC transcriptomes of transgenic relative to wild-type mice; wt ( n = 3), Mm_Foxn4 ( n = 6), and Cm_Foxn4 ( n = 4). ( F ) Localization of B220 + B cells (in green) adjacent to ER-TR7 + mesenchyme (in red). PVS, perivascular space. ( G ) Differential gene expression patterns in TEC transcriptomes of transgenic relative to wild-type mice; wt ( n = 3), Cm_Foxn4 ( n = 4), Cm_Foxn1 ( n = 3), and Cm_dtg ( n = 5). In (C), (E), and (G), each data point represents the average value of at least three mice; all values are significantly different from the wild-type genotype (adjusted P values of <0.05), except those data points falling on “0” Arrows indicate the directions of changes in expression levels between transgenic mice.

Article Snippet: For ER-TR7 B220 staining, the rat anti–ER-TR7 Alexa Fluor 647 Ab (sc-73355 AF647, Santa Cruz Biotechnology) at 1:50 and rat anti-B220 Alexa Fluor 488 Ab (RA3-6B2, eBioscience) at 1:200 were used.

Techniques: Expressing, Binding Assay, Staining, Gene Expression, Transgenic Assay

Journal: Science Advances

Article Title: Retracing the evolutionary emergence of thymopoiesis

doi: 10.1126/sciadv.abd9585

Figure Lengend Snippet: ( A ) Schematic representation of the N-terminal domains of mouse Foxn4, Foxn1, and the Foxn1*4 chimera; boxes correspond to exons, and colored lines correspond to conserved amino acid residues in Foxn4 and Foxn1 clades (see figs. S2 and S8 for details). The > sign denotes the DNA binding and activation domains, which are not shown here. ( B ) Intermediate cellularity of Foxn1*4 thymi (*** P < 0.001; two-tailed t test); wt ( n = 5), Mm_Foxn4 ( n = 6), and Foxn1*4 ( n = 13). ( C ) Enlarged CD4 − CD8 − DN compartment in Foxn1*4 thymi ( P < 0.001; two-tailed t test); wt ( n = 5) and Foxn1*4 ( n = 13). ( D ) Moderately increased numbers of IgM − CD93 + immature B cells ( P = 0.293; two-tailed t test, compared to wt); wt ( n = 3) and Foxn1*4 ( n = 7). ( E ) Foxn1*4 supports intrathymic T cell development ( P = 0.6654; two-tailed t test, compared to wt); wt ( n = 5) and Foxn1*4 ( n = 13). ( F ) Increased B cell development (* P = 0.0335; two-tailed t test, compared to wt); wt ( n = 8), Mm_Foxn4 ( n = 7), and Foxn1*4 ( n = 13). In (E) and (F), data for Mm_Foxn4 transgenics (shaded area) are taken from . ( G ) Flow cytometric analyses of Ly51 expression and UEA1 binding of EpCAM + CD45 − TECs; wt ( n = 5) and Foxn1*4 ( n = 13). ( H ) Epithelial microenvironment of reconstructed thymi resolved by keratin 5 (K5) (in green) and K8 (in red) staining; 4-week-old mice. ( I ) Localization of B220 + B cells (in green) adjacent to ER-TR7 + mesenchyme (in red); inset shows a higher magnification of the indicated region highlighting the perivascular space. ( J and K ) Differential gene expression patterns in TECs; see legend in for details; wt ( n = 3), Mm_Foxn4 ( n = 6), Foxn1*4 ( n = 3), and Cm_dtg ( n = 5). In (B), (E), and (F), each data point represents one mouse.

Article Snippet: For ER-TR7 B220 staining, the rat anti–ER-TR7 Alexa Fluor 647 Ab (sc-73355 AF647, Santa Cruz Biotechnology) at 1:50 and rat anti-B220 Alexa Fluor 488 Ab (RA3-6B2, eBioscience) at 1:200 were used.

Techniques: Binding Assay, Activation Assay, Two Tailed Test, Expressing, Staining, Gene Expression